Journal of Cerebrovascular Disease 2019 Vol.2 No.1 1-32

Hyaluronic Acid-polycaprolactone Nanofibrous Scaffolds: A Possible Device for Nerve Tissue Engineering

Author(s): Feng Xiao1,Jian-Wei Pan1

Affiliation(s): 1Department of Neurosurgery, The First Affiliated Hospital, Zhejiang University, School of Medicine

Corresponding Author: Jian-Wei Pan

Corresponding email(s):

Key Words: Hyaluronic acid; Polycaprolactone; Neural stem cells


Background: To improve the biological compatibility of pure polycaprolactone (PCL) nanofibrous scaffolds by co-spinning of hyaluronic acid (HA) and PCL, and to evaluate the value of HA-PCL scaffolds in nerve tissue engineering by observing the growth of neural stem cells (NSCs) on them.

Methods: Using traditional electrospinning technology, HA and PCL were spun into three kinds of random, aligned, and extremely aligned scaffolds in a ratio of 1:5 and 1:10, and the pure PCL scaffold was prepared at the same time. The sequencing and fiber diameters of various nanofibrous scaffolds were determined by electron microscopy. Then, NSCs were inoculated on the 9 nanofibrous scaffolds, and their proliferation was determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5 diphenyl tetrazolium bromide] assay after 5 days.

Results: Scaffolds of pure PCL, 1:5 HA-PCL, and 1:10 HA-PCL were successfully prepared, whose diameter was about 200 nm and which were divided into random, aligned, and extremely aligned scaffolds by electron microscopy. The MTT assay showed no significant difference in the proliferation of NSCs on 1:10 HA-PCL scaffolds and pure PCL scaffolds. The proliferation of NSCs on 1:5 HA-PCL scaffolds was better than that of 1:10 HA-PCL and pure PCL scaffolds. On the 1:5 HA-PCL scaffold, the more aligned the scaffold is, the faster cells proliferate.

Conclusions: HA can effectively increase the tissue compatibility of PCL scaffolds and promote the proliferation of NSCs. The aligned nanofibrous scaffold is more beneficial to the proliferation of NSCs.

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On-line Access: 2018-12-01

Received: 2018-03-27

Revision Accepted: 2018-09-09

Publish: 2019-03-01

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