Journal of Cerebrovascular Disease 2019 Vol.2 No.1 1-32
Hyaluronic Acid-polycaprolactone Nanofibrous Scaffolds: A Possible Device for Nerve Tissue Engineering
Author(s): Feng Xiao1,Jian-Wei Pan1
Affiliation(s):
1Department of Neurosurgery, The First Affiliated Hospital, Zhejiang University, School of Medicine
Corresponding Author: Jian-Wei Pan
Corresponding email(s): 1202053@zju.edu.cn
Key Words: Hyaluronic acid; Polycaprolactone; Neural stem cells
Abstract:
Background: To improve the biological compatibility of
pure polycaprolactone (PCL) nanofibrous scaffolds by co-spinning of hyaluronic
acid (HA) and PCL, and to evaluate the value of HA-PCL scaffolds in nerve
tissue engineering by observing the growth of neural stem cells (NSCs) on them.
Methods: Using traditional electrospinning
technology, HA and PCL were spun into three kinds of random, aligned, and extremely
aligned scaffolds in a ratio of 1:5
and 1:10, and the pure PCL scaffold was prepared at the same time. The sequencing
and fiber diameters of various nanofibrous scaffolds were determined by
electron microscopy. Then, NSCs were inoculated on the 9 nanofibrous scaffolds,
and their proliferation was determined by MTT [3-(4,5-dimethylthiazol-2-yl)-2,5
diphenyl tetrazolium bromide] assay after 5 days.
Results: Scaffolds of pure PCL, 1:5 HA-PCL, and 1:10
HA-PCL were successfully prepared, whose diameter was about 200 nm and which were
divided into random, aligned, and extremely aligned scaffolds by electron
microscopy. The MTT assay showed no significant difference in the proliferation
of NSCs on 1:10 HA-PCL scaffolds and pure PCL scaffolds. The proliferation of
NSCs on 1:5 HA-PCL scaffolds was better than that of 1:10 HA-PCL and pure PCL scaffolds.
On the 1:5 HA-PCL scaffold, the more aligned the scaffold is, the faster cells
proliferate.
Conclusions: HA can effectively increase the tissue
compatibility of PCL scaffolds and promote the proliferation of NSCs. The aligned
nanofibrous scaffold is more beneficial to the proliferation of NSCs.
Full Text:
On-line Access: 2018-12-01
Received: 2018-03-27
Revision Accepted: 2018-09-09
Publish: 2019-03-01
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