Journal of Cerebrovascular Disease 2018 Vol.1 No.1 1-42

A Simple Procedure for Extracting DNA from Coagulated Blood Samples for DNA Banking

Author(s): Lu-Han Jiang¹,Cheong-Nang Kuok²,Khyati Chandratre²,Grace Meng³,Jia Xu⁴,Yuan-Qiang Lu⁵,San-Ming Wang⁵

Affiliation(s): ¹Faculty of Health Sciences, University of Macau, Macau, China
²Oriental X-Ray Inc, Macau, China
³School of Health Sciences, Macao Polytechnic Institute, Macau, China
⁴Department of Emergency, The First Affiliated Hospital, Zhejiang University, School of Medicine
⁵Department of Emergency Medicine, The First Affiliated Hospital, Zhejiang University, School of Medicine

Corresponding Author: San-Ming Wang

Corresponding email(s): sanmingwang@umac.mo

Key Words: DNA extraction; DNA banking; Coagulated blood

Background: DNA banking is increasingly applied to collect genetic materials for biomedical studies. Traditionally, the source of DNA banking is from the anti-coagulated blood samples as it is easy to isolate cells from the samples for DNA extraction. However, substantial portion of clinical blood samples is coagulated. Because of the difficulty to release blood cells from the clots for DNA extraction, coagulated blood samples are not used for DNA banking.

Methods: In this study, we developed a simple procedure for high-throughput DNA extraction from coagulated blood samples for DNA banking.

Results: We showed that the extracted DNA is the same as the one extracted from anti-coagulated blood samples as reflected by the size distribution and genetic variants across all coding genes.

Conclusions: We developed a simply procedure for high-throughput DNA extraction from coagulated blood cells. Validation results show that the DNA from coagulated blood cells is the same as the DNA from the anti-coagulated blood cells.

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On-line Access: 2018-04-01

Received: 2017-11-05

Revision Accepted: 2018-03-02

Publish: 2018-12-01

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